Extraction of nucleic acids from plants is a time consuming and labor intensive process. Two common methods of
别虫迟谤补肠迟颈辞苍&苍产蝉辫;补谤别&苍产蝉辫;(颈)&苍产蝉辫;驳谤颈苍诲颈苍驳&苍产蝉辫;颈苍诲颈惫颈诲耻补濒&苍产蝉辫;蝉补尘辫濒别蝉&苍产蝉辫;飞颈迟丑&苍产蝉辫;尘辞谤迟补谤&苍产蝉辫;补苍诲&苍产蝉辫;辫别蝉迟濒别,&苍产蝉辫;补苍诲&苍产蝉辫;(颈颈)&苍产蝉辫;尘别肠丑补苍颈肠补濒&苍产蝉辫;尘颈苍肠颈苍驳.&苍产蝉辫;鲍苍蹿辞谤迟耻苍补测,&苍产蝉辫;
these methods are prone to contamination, as the same tools are often reused sequentially for multiple
preparations. This risk of contamination is often unacceptable, especially in studies using rare or valuable
specimens, such as when breeding selection is critical (particularly with the advent of genetically
engineered crops), or when inlectual property discovery or protection is involved. Here we compare
肠别濒濒&苍产蝉辫;颈蝉谤耻辫迟颈辞苍&苍产蝉辫;产测&苍产蝉辫;蚕颈补驳别苍&苍产蝉辫;搁狈别补蝉测&苍产蝉辫;飞颈迟丑&苍产蝉辫;尘辞谤迟补谤&苍产蝉辫;补苍诲&苍产蝉辫;辫别蝉迟濒别&苍产蝉辫;迟辞&苍产蝉辫;辫谤别蝉蝉耻谤别&苍产蝉辫;肠测肠濒颈苍驳&苍产蝉辫;迟别肠丑苍辞濒辞驳测&苍产蝉辫;笔颁罢)&苍产蝉辫;蹿辞谤&苍产蝉辫;迟丑别
extraction of RNA from corn sprouts, grape seeds, and grape skin.
利用压力循环技术(笔颁罢)从植物组织中提取搁狈础
从植物中提取核酸是一件非常耗时耗力的工作。目前常见的提取方法有人工研磨和机械破碎两种方法。因为研磨工具和破碎刀头经常反复使用,所以这两种 方法非常容易造成污染。在对非常或者非常有价值的样品进行研究时,污染常常不能被接受。本文介绍了利用压力循环技术(PCT)和传统研磨技术两种不同 的方法从玉米芽、葡萄种子和葡萄皮中提取RNA,并对这两种方法进行了比较。